Journal
/
/
Celle sammenlægning Assays til at evaluere bindingen af Drosophila Notch med Trans-ligander og dens hæmning af Cis-ligander
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Cell Aggregation Assays to Evaluate the Binding of the Drosophila Notch with Trans-Ligands and its Inhibition by Cis-Ligands
DOI:

05:48 min

January 02, 2018

,

Chapters

  • 00:05Title
  • 00:38Assessment of Binding Between Notch Receptor and Trans-ligands
  • 01:47Aggregation Between Signal-sending and Signal-receiving Cells
  • 03:11Evaluation of Inhibition of Binding Between Notch and Trans-ligands by Cis-ligands
  • 04:15Results: Faithful Analysis of Notch Signaling
  • 05:30Conclusion

Summary

Automatic Translation

I vivo systemers kompleksitet gør det vanskeligt at skelne mellem aktivering og hæmning af Notch receptor af trans- og cis-ligander, henholdsvis. Vi præsenterer her, en protokol baseret på in vitro- celle-sammenlægning assays til kvalitative og semi-kvantitative evaluering af bindingen af Drosophila hak til trans-ligander vs cis-ligander.

Related Videos

Read Article