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Imaging Approaches to Assessments of Toxicological Oxidative Stress Using Genetically-encoded Fluorogenic Sensors
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1Department of Environmental Sciences and Engineering, Gillings School of Global Public Health,University of North Carolina at Chapel Hill, 2Environmental Public Health Division,National Health and Environmental Effects Research Laboratory, U.S. Environmental Protection Agency, 3Oak Ridge Institute for Science and Education
Chapters
- 00:04Title
- 00:40Microscopy Set-up
- 05:09Data Acquisition
- 07:36Results: Toxicological Assessment of Changes in the Intracellular EGSH as Measured by roGFP2
- 08:54Conclusion
This manuscript describes the use of genetically-encoded fluorogenic reporters in an application of live-cell imaging for the examination of xenobiotic-induced oxidative stress. This experimental approach offers unparalleled spatiotemporal resolution, sensitivity, and specificity while avoiding many of the shortcomings of conventional methods used for the detection of toxicological oxidative stress.
