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Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!
JoVE Journal
Chemistry
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JoVE Journal Chemistry
Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

Visualizing Lignification Dynamics in Plants with Click Chemistry: Dual Labeling is BLISS!

DOI:
10.3791/56947-v

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10:40 min

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January 26, 2018

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, , ,
1UGSF – Unité de Glycobiologie Structurale et Fonctionnelle, CNRS, UMR 8576,Université de Lille

Chapters

  • 00:05Title
  • 01:18Metabolic Incorporation of Chemical Reporters
  • 03:29Dual Fluorescence Labeling of Plant Cross-section Samples by SPAAC and CuAAC
  • 06:08Mounting of Samples on Microscope Slides
  • 07:49Results: Study of Lignification Dynamics via BLISS
  • 09:40Conclusion

Summary

Automatic Translation

Automatic Translation

BLISS, a dual labeling protocol for studying lignification dynamics, was developed. Using synthetic monolignol reporters and a sequential combination of SPAAC and CuAAC bioorthogonal click reactions, this methodology paves the way to in-depth analysis of the factors that regulate the biogenesis of lignins in planta.

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