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Chapters
- 00:05Title
- 01:09Preparation of CRISPR-reagents for Embryo Microinjection
- 04:03Microinjection of CRISPR-components into Zebrafish Embryos
- 06:12Analysis of Efficiency of Indel Formation Using an HMA
- 08:45Identification and Propagation of Knock-out Lines
- 09:45Results: Zebrafish Embryos Exhibit a Pigment Defect when Injected with an sgRNA Targeting Tyrosinase at the One Cell Stage
- 10:32Conclusion
Målrettet genom-redigering i modelsystem Danio rerio (zebrafisk) er blevet stærkt fremmet af fremkomsten af CRISPR-baserede tilgange. Heri, beskriver vi en strømlinet, robust protokol for generation og identifikation af CRISPR-afledte nonsens alleler, der inkorporerer heteroduplex mobilitet analyse og identifikation af mutationer ved hjælp af næste generation sequencing.
