/

/

金小蜂金小蜂基因组操作的胚胎显微注射和移植技术

JoVE Journal
Genetics

This content is Free Access.

JoVE Journal Genetics

Embryo Microinjection and Transplantation Technique for Nasonia vitripennis Genome Manipulation

Please note that all translations are automatically generated. Click here for the English version.

金小蜂金小蜂基因组操作的胚胎显微注射和移植技术

DOI:

10.3791/56990-v

•

09:05 min

•

December 25, 2017

•

Ming Li², Michelle Bui², Omar S. Akbari²

¹Department of Entomology and Riverside Center of Disease Vector Research, Institute for Integrative Genome Biology,University of California, Riverside, ²Section of Cell and Developmental Biology, Division of Biological Sciences,University of California, San Diego

Chapters

00:05Title
00:47N. vitripennis Colony Rearing
01:40Collection and Alignment of N. vitripennis Pre-blastoderm Stage Embryos
04:13Embryo Microinjection
05:58Transplanting Injected G0 N. vitripennis Embryos onto Pre-stung Host
07:15Results: sgRNA and Cas9 Microinjection in N. vitripennis Transplanted Embryos
08:23Conclusion

Summary

Automatic Translation

显微注射的金小蜂金小蜂胚胎是产生遗传基因组改变的必要方法。这里描述的是一个详细的程序, 显微注射和移植的金小蜂金小蜂胚胎, 这将大大促进未来的基因组操纵在这个有机体。

Tags

Keywords: Embryo Microinjection Nasonia Vitripennis Genome Manipulation Venom Production Sex Determination Wasp Colonies Blowfly Pupae Parasitization Embryo Collection Embryo Alignment Slide Microinjection Technique

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

核酸的传递通过显微注射胚胎在世界范围内的农业害虫，<em>地中海实蝇</em

单细胞基因表达谱使用流式细胞仪和qPCR内部标准

使用生物素化补骨脂素全球定位RNA-RNA相互作用

G2-seq: 一种基于高通量测序技术的基因组晚期复制区域识别方法

单缓步动物标本的超低输入基因组测序库制备

全基因组对真核生物转录误差的监测

利用 Crispr-ca 在哺乳动物细胞系中进行基因组编辑

转化、基因组编辑和拟合氮的热带大麻树帕拉斯波尼亚安德森

以乌苏图病毒为例，验证全基因组纳米孔测序

胚胎微注射技术，用于在库莱克斯五角星高效位点特异性突变体

Read Article