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Imunoprecipitação da cromatina nativa usando Neurospheres de Tumor de cérebro murino
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1Department of Cell and Developmental Biology,University of Michigan Medical School, 2Department of Neurosurgery,University of Michigan Medical School, 3Cancer Research Summer Internship Program (CARSIP), Cancer Biology Program,University of Michigan Medical School, 4Department of Biology,University of Puerto Rico-Río Piedras Campus
Chapters
- 00:05Title
- 00:40Generation of Brain Tumor Neurospheres and Culturing Conditions
- 02:23Chromatin Preparation
- 04:52Immunoprecipitation (IP)
- 08:21Results: Representative ChIP qPCR Data as Percent Input
- 09:05Conclusion
Mecanismos epigenéticos são frequentemente alterados no glioma. Imunoprecipitação da cromatina pode ser usada para estudar as consequências de alterações genéticas no glioma que resultam de mudanças nas modificações do histone que regulam a transcrição de gene e a estrutura da cromatina. Este protocolo descreve imunoprecipitação da cromatina nativa na neurospheres de tumor murino de cérebro.
