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使用河豚毒素耐药性钠通道检查果蝇中的 Monosynaptic 连接

JoVE Journal
Neuroscience

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JoVE Journal Neuroscience

Examining Monosynaptic Connections in Drosophila Using Tetrodotoxin Resistant Sodium Channels

Please note that all translations are automatically generated. Click here for the English version.

使用河豚毒素耐药性钠通道检查果蝇中的 Monosynaptic 连接

DOI:

10.3791/57052-v

•

09:55 min

•

February 14, 2018

•

Xiaonan Zhang, Quentin Gaudry

¹Department of Biology,University of Maryland

Chapters

00:04Title
00:42Preparation
03:17Obtaining a Whole-cell Recording Configuration
05:36Testing for Synaptic Connectivity
07:17Results: Using Tetrotoxin Engineered Resistance for Probing Synapses (TERPS)
08:58Conclusion

Summary

Automatic Translation

本文的特点是通过使用河豚毒素和河豚毒素耐药钠通道, NaChBac 来检测神经元之间的 monosynaptic 连接。

Tags

Keywords: Monosynaptic Connections Drosophila Tetrodotoxin Resistant Sodium Channels Electrophysiology Dissection Patch-clamp Recordings Recording Saline CsChrimson All-trans-Retinal Antennal Lobes Glial Sheathing

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