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基于芯片的数字 PCR 检测新鲜冷冻胃癌组织中的一个CDH1罕见转录变体

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Cancer Research

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JoVE Journal Cancer Research

Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR

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基于芯片的数字 PCR 检测新鲜冷冻胃癌组织中的一个CDH1罕见转录变体

DOI:

10.3791/57066-v

•

09:16 min

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February 05, 2018

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Chiara Molinari, Raefa Abou Khouzam, Samanta Salvi, Tania Rossi, Guglielmina Nadia Ranzani, Daniele Calistri

¹Biosciences Laboratory,Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST) IRCCS, ²Department of Biology and Biotechnology,University of Pavia

Chapters

00:04Title
01:03Preparing the PCR Reaction Mixes and the PCR Chip
04:31Digital PCR (dPCR)
05:30Chip Analysis
07:05Results: Screening for CDH1a Expression in Tumor Tissue
08:28Conclusion

Summary

Automatic Translation

该手稿描述了一种基于芯片的数字 PCR 方法, 用于检测从胃癌患者中获得的新鲜冰冻正常和肿瘤组织中罕见的CDH1转录变体 (CDH1a)。

Tags

Keywords: CDH1 Rare Transcript Variant Digital PCR Chip-based Low-abundant Targets CDNA Quantitative PCR Biological Samples Master Mix Assay No Template Control Primer Centrifugation Chip Loader Immersion Fluid Loading Blade

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