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Chromatin Immunoprecipitation (ChIP) Histone endringer fra Saccharomyces cerevisiae
JoVE Journal
Genetics
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JoVE Journal Genetics
Chromatin Immunoprecipitation (ChIP) of Histone Modifications from Saccharomyces cerevisiae
DOI:

11:06 min

December 29, 2017

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Chapters

  • 00:05Title
  • 00:52In Vivo Crosslinking of Proteins to DNA
  • 02:19Making Yeast Lysates
  • 04:08Immunoprecipitation (IP) of Modified Histones
  • 05:11Washing IPs, Eluting Histone-DNA Complexes and Reversing Protein-DNA Crosslinks
  • 07:24Quantitative PCR (qPCR) to Detect Enriched Genomic Regions
  • 08:46Results: ChIP Reveals Histone Modification Status at ant Genomic Region
  • 10:17Conclusion

Summary

Automatic Translation

Her beskriver vi en protokoll for chromatin immunoprecipitation av modifisert histones fra spirende gjær Saccharomyces cerevisiae. Immunoprecipitated DNA blir brukt i kvantitative PCR avhøre overflod og lokalisering av histone post-translasjonell endringer i genomet.

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