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Journal
/
Behavior
/
用于检测
果蝇
幼虫缺氧的挖洞/隧道法
JoVE Journal
Behavior
This content is Free Access.
JoVE Journal
Behavior
A Burrowing/Tunneling Assay for Detection of Hypoxia in
Drosophila melanogaster
Larvae
Please note that all translations are automatically generated.
Click here for the English version.
用于检测
果蝇
幼虫缺氧的挖洞/隧道法
DOI:
10.3791/57131-v
•
09:04 min
•
March 27, 2018
•
Karen M. Qiang
2
,
Fanli Zhou
,
Kathleen M. Beckingham
1
Department of Biosciences
,
Rice University
,
2
Yale Medical School
Chapters
00:04
Title
01:03
Preparation of the Larvae and Assay Plates
02:52
Conducting the Assay
04:24
Preparing Assay Plates for Imaging
07:06
Results: Assessing RNAi Suppression of
uninflatable
08:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该议定书描述了一种简单的检测方法, 用于识别在正常大气氧水平下缺氧的
果蝇
幼虫。该协议允许低氧幼虫与其他突变体区别, 这些变种显示重叠的表型, 如迟缓或缓慢生长。
Tags
Keywords: Burrowing Assay
Tunneling Assay
Hypoxia Detection
Drosophila Melanogaster Larvae
Oxygen Deficiency
Larval Behavior
Trachea
Wandering Phase
Genetic Crosses
Egg Collection
Agar Plates
Nipagen
Yeast Paste
Cork Board
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