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Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes
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Biochemistry
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JoVE Journal Biochemistry
Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

Determining if DNA Stained with a Cyanine Dye Can Be Digested with Restriction Enzymes

DOI:
10.3791/57141-v

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06:58 min

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February 02, 2018

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, , , , ,
1Department of Chemistry,University of Nebraska – Kearney

Chapters

  • 00:05Title
  • 00:42Preparation of Dyes, Buffers, and Agarose Gel
  • 01:34Preparation of Stained DNA and Restriction Enzyme Assay
  • 02:47Electroeluting Stained DNA in an Agarose Gel
  • 04:44Results: Digestion of Cyanine Dye-treated Lambda DNA
  • 06:08Conclusion

Summary

Automatic Translation

Automatic Translation

Staining DNA molecules for fluorescence microscopy allows a scientist to view them during an experiment. In the method presented here, DNA molecules are pre-stained with fluorescent dyes and digested with methylation and non-methylation sensitive restriction enzymes.

Tags

Keywords: DNACyanine DyeRestriction EnzymesDigestionMethylationGenomicsAgarose GelLambda DNATE BufferRestriction EnzymeEDTAGel ElectrophoresisBufferWater BathIncubation

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