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Biochemistry

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JoVE Journal Biochemistry
Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells
 

Analyses of Mitochondrial Calcium Influx in Isolated Mitochondria and Cultured Cells

Article DOI: 10.3791/57225-v • 08:29 min • April 27th, 2018
April 27th, 2018 •
1, 1, 2, 1
1Department of Pediatrics, Emory University School of Medicine, 2Emory College of Arts and Sciences, Emory University

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Summary
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Here, we present two protocols for the measurement of mitochondrial Ca2+ influx in isolated mitochondria and cultured cells. For isolated mitochondria, we detail a plate reader-based Ca2+ import assay using the Ca2+ sensitive dye calcium green-5N. For cultured cells, we describe a confocal microscopy method using the Ca2+ dye Rhod-2/AM.

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Keywords: Mitochondrial Calcium Influx Calcium Signaling Mitochondrial Calcium Dynamics Disease Conditions Plate-reader-based Mitochondrial Calcium Uptake Confocal-microscope-based Mitochondrial Calcium Influx Isolated Heart MSEGTA Buffer Calcium Green-5N Calcium Chloride
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