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三维肽支架中哺乳动物细胞的培养

JoVE Journal
Bioengineering

This content is Free Access.

JoVE Journal Bioengineering

Culturing Mammalian Cells in Three-dimensional Peptide Scaffolds

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三维肽支架中哺乳动物细胞的培养

DOI:

10.3791/57259-v

•

07:52 min

•

June 13, 2018

•

Nausika Betriu, Lourdes Recha-Sancho, Carlos E. Semino²

¹Tissue Engineering Research Laboratory, Department of Bioengineering, IQS-School of Engineering,Ramon Llull University, ²Hebe Biolab

Chapters

00:05Title
00:51Self-Assembling Peptide Scaffold (SAPS) Preparation
01:39Cell Suspension Preparation
02:323D Cell Encapsulation into SAPS
04:52Cell Viability in 3D-SAPS
05:55Results: Attaining a 3D-Culture System in Self Assembling Peptide Scaffolds
07:03Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个协议, 以获得 3 d-培养系统的自组装肽支架, 以促进分化去分化肉瘤人关节软骨细胞成软骨样组织。

Tags

Keywords: 3D Cell Culture Peptide Scaffold RAD16-I Cell-cell Interaction Cell-matrix Interaction Cell Proliferation Cell Migration Cell Differentiation Regenerative Medicine Tumor Models Diabetes Neurodegenerative Diseases Cell Suspension Cell Concentration Sonication Centrifugation

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