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一种基于流式细胞仪的检测方法, 用于识别破坏荧光标记的 CXC 趋化因子配体12到 CXC 趋性因子受体4的化合物
JoVE Journal
Biology
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JoVE Journal Biology
A Flow Cytometry-based Assay to Identify Compounds That Disrupt Binding of Fluorescently-labeled CXC Chemokine Ligand 12 to CXC Chemokine Receptor 4
DOI:

06:56 min

March 10, 2018

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Chapters

  • 00:04Title
  • 00:54Competition Binding Assay
  • 03:00Analysis of the Samples by Flow Cytometry
  • 05:35Results: Evaluation and Analysis of Active and Inactive Compounds in the Competition Binding Assay
  • 06:35Conclusion

Summary

Automatic Translation

本文介绍了一种基于流式细胞术的细胞结合检测方法, 主要用作筛选工具, 用于识别抑制荧光标记为 CXC 趋化因子配体 12 (CXCL12) 的化合物对 CXC 趋化因子受体 4 (CXCR4) 的束缚。

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