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Immunology and Infection
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Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy
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Immunology and Infection
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JoVE Journal Immunology and Infection
Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

Preparation of Murine Submandibular Salivary Gland for Upright Intravital Microscopy

DOI:
10.3791/57283-v

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08:13 min

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May 07, 2018

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, , ,
1Theodor-Kocher Institute,University of Bern, 2Center for Infectious Diseases, Integrative Virology,University Clinic of Heidelberg

Chapters

  • 00:05Title
  • 00:39Removal of the Fur and Fixation of the Mouse on the Stage
  • 02:46Surgical Exposure of the Salivary Gland Using a Stereomicroscope
  • 04:09Fixing of the Gland, Heating Ring, and the Temperature Probe
  • 06:10Results: Use of Immunofluorescence Imaging to Study the Submandibular Gland
  • 07:19Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a protocol to surgically expose and stabilize the murine submandibular salivary gland for intravital imaging using upright intravital microscopy. This protocol is easily adaptable to other exocrine glands of the head and neck region of mice and other small rodents.

Tags

Keywords: Murine Submandibular Salivary GlandUpright Intravital MicroscopySurgical PreparationImmunologyC57 Black Six MouseHair Removal CreamCoverslip HolderEar BarSurgical TapeAngled Curved ForcepsHeating LampSubmandibular Salivary Gland

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