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Kvantitative og kvalitative metode for Sphingomyelin af LC-MS bruger to stabile brændselsfremstilling mærket Sphingomyelin arter
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Quantitative and Qualitative Method for Sphingomyelin by LC-MS Using Two Stable Isotopically Labeled Sphingomyelin Species
DOI:

08:53 min

May 07, 2018

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Chapters

  • 00:05Title
  • 00:33Preparation of Lipid Samples
  • 03:06Sample Preparation for Calibration Curve and Method Validation
  • 03:50SM Analysis by LC-ESI-MS/MS
  • 05:33Batch File Creation and Assigning the MS Three Product Ion Spectra of Sphingomyelin
  • 06:23Quantitative Analysis of SM by LC-ESI-MS/MS
  • 07:02Results: Validating the Quantitative and Qualitative Method for Sphingomyelin
  • 08:01Conclusion

Summary

Automatic Translation

Her præsenterer vi en protokol til at kvantificere og kvalificere hver sphingomyelin arter ved hjælp af flere reaktion overvågning og MS/MS/MS tilstand, henholdsvis.

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