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96井格式人诱导多潜能干细胞源神经元高速拉伸损伤的方法
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Method for High Speed Stretch Injury of Human Induced Pluripotent Stem Cell-derived Neurons in a 96-well Format
DOI:

08:35 min

April 20, 2018

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Chapters

  • 00:05Title
  • 00:42Plate Fabrication
  • 02:02Indenter Block Alignment
  • 04:29Plate Stretching
  • 05:23Membrane Stretch Characterization and Cell Culture Injury
  • 06:58Results: Representative Cell Culture Injury Analyses
  • 08:07Conclusion

Summary

Automatic Translation

在这里, 我们提出了一个人的体外模型的拉伸损伤96井格式的时间刻度与撞击创伤。这包括制造可伸缩板的方法, 量化的机械侮辱, 培养和损伤细胞, 成像, 高含量分析, 以量化损伤。

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