Journal
/
Medicine
/
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

Characterization of Immune Cells in Human Adipose Tissue by Using Flow Cytometry

DOI:
10.3791/57319-v

•

07:32 min

•

March 06, 2018

•

, , , , ,
1Department of Internal Medicine,MUMC, 2CARIM,MUMC, 3Department of Immunology and Biochemistry, Biomedical Research Institute,Hasselt University, 4Department of Pathology,MUMC

Chapters

  • 00:04Title
  • 01:06Stromal Vascular Fraction (SVF) Adipose Tissue (AT) Isolation
  • 02:42SVF Staining and Flow Cytometry Analysis
  • 04:50Results: Representative Visceral AT Immune Cell Abundance
  • 06:33Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a method to analyze immune cell content of adipose tissue by isolation of immune cells from adipose tissue and subsequent analysis using flow cytometry.

Tags

Flow CytometryStromal Vascular FractionImmune CellsPhenotypingMetabolic ResearchObesityInflammationCell IsolationCell Staining

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57319?article_type=v

Isolation, Characterization and Comparative Differentiation of Human Dental Pulp Stem Cells Derived from Permanent Teeth by Using Two Different Methods
check_url/57319?article_type=v

High-throughput Flow Cytometry Cell-based Assay to Detect Antibodies to N-Methyl-D-aspartate Receptor or Dopamine-2 Receptor in Human Serum
check_url/57319?article_type=v

Stereological and Flow Cytometry Characterization of Leukocyte Subpopulations in Models of Transient or Permanent Cerebral Ischemia
check_url/57319?article_type=v

Human Brown Adipose Tissue Depots Automatically Segmented by Positron Emission Tomography/Computed Tomography and Registered Magnetic Resonance Images
check_url/57319?article_type=v

Studying Pancreatic Cancer Stem Cell Characteristics for Developing New Treatment Strategies
check_url/57319?article_type=v

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
check_url/57319?article_type=v

Assessment of Human Adipose Tissue Microvascular Function Using Videomicroscopy
check_url/57319?article_type=v

Whole Body and Regional Quantification of Active Human Brown Adipose Tissue Using 18F-FDG PET/CT
check_url/57319?article_type=v

Flow Cytometry Analysis of Immune Cell Subsets within the Murine Spleen, Bone Marrow, Lymph Nodes and Synovial Tissue in an Osteoarthritis Model
check_url/57319?article_type=v

Fat-Covered Islet Transplantation Using Epididymal White Adipose Tissue

Read Article