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En murin bukspottskörteln Islet Cell-baserad Screening för diabetogent miljö kemikalier
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Murine Pancreatic Islet Cell-based Screening for Diabetogenic Environmental Chemicals
DOI:

07:39 min

June 25, 2018

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Chapters

  • 00:04Title
  • 00:49Pancreas Perfusion and Removal
  • 01:43Pancreas Digestion and Purification of Islets
  • 03:30Plating and Treatment of Islet Cells
  • 04:23ROS Staining and Measurement
  • 05:28Results: Xenobiotic Treatment-induced Reactive Oxygen Species and Inhibition of Insulin Secretion in Isolated Pancreatic Islet Cells
  • 06:39Conclusion

Summary

Automatic Translation

Här presenterar vi ett protokoll för att isolera mus bukspottkörtelns cellöar för screening av ROS induktioner av xenobiotika för att identifiera potentiella diabetogent xenobiotiska kemikalier.

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