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Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining
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Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

Quantification of Lipid Abundance and Evaluation of Lipid Distribution in Caenorhabditis elegans by Nile Red and Oil Red O Staining

DOI:
10.3791/57352-v

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07:46 min

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March 05, 2018

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, , ,
1Leonard Davis School of Gerontology,University of Southern California, 2Molecular & Computational Biology Section,University of Southern California, 3Integrative Program for Biological & Genome Sciences,University of North Carolina at Chapel Hill

Chapters

  • 00:05Title
  • 00:34Nile Red Staining of Lipids
  • 03:42Oil Red O Staining of Lipids
  • 05:28Results: Lipid Quantification and Distribution in C. elegans by Nile Red and Oil Red O Staining
  • 06:53Conclusion

Summary

Automatic Translation

Automatic Translation

Nile red staining of fixed Caenorhabditis elegans is a method for quantitative measurement of neutral lipid deposits, while oil red O staining facilitates qualitative assessment of lipid distribution among tissues.

Tags

Lipid AbundanceLipid DistributionCaenorhabditis ElegansNile RedOil Red OLipid MetabolismLipid HomeostasisOP50 E.coliPBSTIsopropanolNR Working SolutionFITC GFP Channel

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