Journal
/
Immunology and Infection
/
Byggingen av syntetisk Phage vises Fab biblioteket med skreddersydd mangfold
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Construction of Synthetic Phage Displayed Fab Library with Tailored Diversity

Byggingen av syntetisk Phage vises Fab biblioteket med skreddersydd mangfold

DOI:
10.3791/57357-v

•

12:31 min

•

May 01, 2018

•

, , , , ,
1Laboratory of Antibody Engineering, Shanghai Institute for Advanced Immunochemical Studies,ShanghaiTech University, 2Banting and Best Department of Medical Research, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto, 3Department of Molecular Genetics, Terrence Donnelly Center for Cellular and Biomolecular Research,University of Toronto

Chapters

  • 00:04Title
  • 01:18Electroporation and Calculation of the Library Size
  • 06:02Quality Assessment by Protein A/L Direct Binding ELISA Assay and Sequencing
  • 09:43Results: Conversion of dU-ssDNA to CCC-DNA and Phage Titration for Calculation of Library Size
  • 11:07Conclusion

Summary

Automatic Translation

Automatic Translation

Denne protokollen beskriver en detaljert fremgangsmåte for bygging av en phage vises syntetiske antistoffer biblioteket med skreddersydd mangfold. Syntetisk antistoffer har bred programmer fra grunnforskning sykdom diagnostikk og therapeutics.

Tags

Keywords: Synthetic Phage DisplayFab LibraryAntibody Library ConstructionAntibody DiversityMonoclonal Antibody DevelopmentPhage Display TechnologyKunkel MutagenesisElectroporationDiversity EvaluationELISA

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57357?article_type=v

'Bioluminescent' Reporter Phage for the Detection of Category A Bacterial Pathogens
check_url/57357?article_type=v

Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
check_url/57357?article_type=v

Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
check_url/57357?article_type=v

Following Cell-fate in E. coli After Infection by Phage Lambda
check_url/57357?article_type=v

Imaging av HIV-1-konvolutt-indusert virologisk Synapse og signaliserer på Syntetiske Lipid bilayers
check_url/57357?article_type=v

Skalerbar høy gjennomstrømning Utvalg fra fag-vises Syntetiske antistoffbiblioteker
check_url/57357?article_type=v

Fag Phenomics: Fysiologiske tilnærminger for å karakter Novel virale proteiner
check_url/57357?article_type=v

Analyse av lymfocytt-ekstravasasjon ved hjelp av en<em> In Vitro</em> Modell av det menneskelige blod-hjernebarrieren
check_url/57357?article_type=v

Overlappende peptid biblioteket tilordne Qa-1 Epitopes i et Protein
check_url/57357?article_type=v

Replikering av bestilt, Nonredundant bibliotek av Pseudomonas aeruginosa belastning PA14 Transposon innsetting mutanter

Read Article