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A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
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Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum

DOI:
10.3791/57371-v

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07:06 min

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April 10, 2018

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,
1Department of Microbiology and Immunology,Uniformed Services University

Chapters

  • 00:04Title
  • 00:47Day One: Infection of K562 Cells with DENV in the Presence of ZIKV Convalescent
  • 03:53Day 3: Fixation and Flow Cytometry
  • 04:51Data Analysis
  • 05:31Results: Antibody-dependent Enhancement of Dengue Virus
  • 06:31Conclusion

Summary

Automatic Translation

Automatic Translation

We describe a simple and easy protocol for measuring antibody dependent enhancement of infection by Zika virus convalescent serum using Dengue virus Reporter Viral Particles.

Tags

Flow CytometryAntibody Dependent EnhancementDengue VirusZika VirusConvalescent SerumRhesus MacaquesIn VitroVirus InfectionRPMISerial DilutionsDengue Virus SerotypesK562 CellsTrypan BlueHemocytometerCentrifugation

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