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En enkel flowcytometri basert analyse for å fastslå In Vitro antistoff avhengige forbedring av Dengue Virus benytter Zika Virus rekonvalesent Serum
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A Simple Flow Cytometry Based Assay to Determine In Vitro Antibody Dependent Enhancement of Dengue Virus Using Zika Virus Convalescent Serum
DOI:

07:06 min

April 10, 2018

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Chapters

  • 00:04Title
  • 00:47Day One: Infection of K562 Cells with DENV in the Presence of ZIKV Convalescent
  • 03:53Day 3: Fixation and Flow Cytometry
  • 04:51Data Analysis
  • 05:31Results: Antibody-dependent Enhancement of Dengue Virus
  • 06:31Conclusion

Summary

Automatic Translation

Vi beskriver en enkel og enkel protokoll for å måle antistoff avhengige forbedring av infeksjon av Zika virus rekonvalesent serum ved bruk av Dengue virus Reporter viruspartikler.

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