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Immunology and Infection

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Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

JoVE Journal
Immunology and Infection

This content is Free Access.

JoVE Journal Immunology and Infection

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

Visual and Microscopic Evaluation of Streptomyces Developmental Mutants

DOI:

10.3791/57373-v

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08:42 min

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September 12, 2018

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Jennifer A. Bennett, Garrett V. Kandell, Sean G. Kirk, Joseph R. McCormick

¹Department of Biology and Earth Science, Biochemistry and Molecular Biology Program,Otterbein University, ²Department of Biological Sciences,Duquesne University

Chapters

00:04Title
01:19Propagation of Streptomyces and Comparing and Recording Visual Appearance
03:02Phase-contrast Microscopy
04:40Fluorescence Microscopy
06:10Results: Visual and Microscopic Analysis of Streptomyces Developmental Mutants
07:34Conclusion

Summary

Automatic Translation

Here we present protocols for novice researchers to initiate phenotyping for the pharmacologically important bacterial genus Streptomyces.

Tags

Streptomyces Phenotypic Observation Visual Characterization Microscopic Examination Bacterial Propagation Storage Streptomyces Coelicolor Actinomyces High School Classroom Undergraduate Teaching Laboratory Colony Morphology Mutagenesis Wild Type Mutant Strains Agar Plate Streaking Incubation Sterile Technique Contamination Prevention

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