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Isolement des cellules endothéliales humaines du côlon Normal et carcinome Colorectal - un protocole amélioré
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1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
Chapters
- 00:04Title
- 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
- 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
- 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
- 08:59Conclusion
Cellules endothéliales tumorales sont des déterminants importants du microenvironnement tumoral et l’évolution de la maladie. Ici, un protocole pour l’isolement des cellules endothéliales pures et viables de carcinome colorectal humain et côlon normal pour être utilisé dans la recherche de tests et la pathogenèse est décrite.
