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Isolering av menneskelig endotelceller fra Normal kolon og Colorectal Carcinoma - en forbedret protokoll
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1Division of Molecular and Experimental Surgery, Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 2Department of Surgery, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 3Division of Nephropathology, Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg, 4Department of Pathology, University Medical Center Erlangen,Friedrich-Alexander University of Erlangen-Nuremberg
Chapters
- 00:04Title
- 00:51Surgery, Preparation of Tissue, and Generation of Single Cell Suspension
- 04:17FACS Sorting, Harvesting and Characterization of Endothelial Cells
- 07:17Results: Isolation of Normal and Tumor Endothelial Cells via FACS Sorting and Study of Endothelial Cell Specific Markers
- 08:59Conclusion
Svulst endotelceller er viktig determinanter av svulst microenvironment og løpet av sykdommen. Her, er en protokoll for isolering av ren og levedyktig endotelceller fra menneskelige colorectal karsinom og normal kolon i narkotika testing og patogenesen forskning beskrevet.
