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斑马鱼脑幼虫神经元、巨噬细胞和小胶质的分离和 RNA 提取
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Isolation and RNA Extraction of Neurons, Macrophages and Microglia from Larval Zebrafish Brains
DOI:

09:20 min

April 27, 2018

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Chapters

  • 00:04Title
  • 01:05Homogenization
  • 04:40Microglia Immunostaining
  • 06:41Results: Isolation of Neurons, Macrophages, and Microglia, and their RNA from Larval Zebrafish Brains
  • 08:25Conclusion

Summary

Automatic Translation

我们提出了一种在生理和病理条件下, 从斑马鱼脑中分离出神经元、巨噬细胞和小胶质的协议。分离后, 从这些细胞中提取 RNA, 分析其基因表达谱。该协议允许收集高质量的 RNA 来执行下游分析, 如 qPCR 和转录组学。

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