Journal
/
Genetics
/
小鼠卵母细胞在减数分裂过程中对人类基因功能的评估
JoVE Journal
Genetics
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Genetics
Using Mouse Oocytes to Assess Human Gene Function During Meiosis I
Please note that all translations are automatically generated. Click here for the English version.

小鼠卵母细胞在减数分裂过程中对人类基因功能的评估

DOI:
10.3791/57442-v

•

11:13 min

•

April 10, 2018

•

, , ,
1IVI-RMA New Jersey, 2Jefferson College of Biomedical Sciences,Thomas Jefferson University, 3Department of Genetics,Rutgers, The State University of New Jersey

Chapters

  • 00:04Title
  • 01:00Molecular Cloning
  • 02:23Kinetochore-microtubule Attachment Assay
  • 04:57Chromosome Alignment Challenge Assay
  • 08:52Results: Assessment of Human Gene Function During Meiosis in Mouse Oocytes
  • 10:12Conclusion

Summary

Automatic Translation

Automatic Translation

随着与人类疾病相关的遗传变异开始显露出来, 开发系统以迅速评估那些被确定的变种的生物学意义变得越来越重要。本协议描述了在女性减数分裂过程中使用小鼠卵母细胞评估人类基因功能的方法。

Tags

Keywords: Mouse OocytesHuman Gene FunctionMeiosis IMaternal AneuploidyIn Vitro FertilizationExome SequencingCloningMutagenesisIn Vitro TranscriptionMicroinjectionMilrinoneMetaphase I

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57442?article_type=v

角膜上皮伤口愈合过程中的热休克蛋白27的功能为基础的RNA干扰研究
check_url/57442?article_type=v

单细胞基因表达利用多重RT-qPCR的表征罕见的淋巴人群的异质性
check_url/57442?article_type=v

逆转录病毒扫描：映射MLV集成站点以定义细胞特异性监管区域
check_url/57442?article_type=v

CRISPR/Cas9 和 SsODN 在人细胞中催化点突变修复功能的标准方法研究
check_url/57442?article_type=v

量化的信息编码的基因表达水平在寿命调制下线虫的宽范围饮食限制
check_url/57442?article_type=v

CRISPR/Cas9 对小鼠和人造血祖细胞的高效基因破坏
check_url/57442?article_type=v

CRISPR/Cas9 基因编辑制作人类疟疾寄生虫的条件突变体
check_url/57442?article_type=v

用小鼠脾评估病原体相关分子模式对时钟基因表达的影响
check_url/57442?article_type=v

墨西哥鱼基因功能的操纵
check_url/57442?article_type=v

使用基因激活 crispr 过度表达长非编码 rna

Read Article