/

Developmental Biology

/

CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法

JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Developmental Biology

CARIP-Seq and ChIP-Seq: Methods to Identify Chromatin-Associated RNAs and Protein-DNA Interactions in Embryonic Stem Cells

Please note that all translations are automatically generated. Click here for the English version.

CARIP 和芯片序列: 鉴定胚胎干细胞中染色质相关 rna 和蛋白质-DNA 相互作用的方法

DOI:

10.3791/57481-v

•

11:13 min

•

May 25, 2018

•

Benjamin L. Kidder²

¹Department of Oncology,Wayne State University School of Medicine, ²Karmanos Cancer Institute,Wayne State University School of Medicine

Chapters

00:04Title
01:02Crosslinking of Embryonic Stem Cells for ChIP and CARIP
02:29Chromatin Sonication for ChIP and CARIP
03:48ChIP and CARIP Process
07:12Double-stranded cDNA Synthesis for CARIP-Seq
08:53Use of ChIP-Seq and CARIP-Seq to Study Global Protein-DNA Interactions and Chromatin-Associated RNAs in Embryonic Stem Cells
10:43Conclusion

Summary

Automatic Translation

在这里, 我们描述了执行芯片序列和 CARIP 的方法, 包括用于下一代测序的库准备, 以生成 ES 细胞中的全球 epigenomic 和染色质相关的 RNA 图谱。

Tags

Keywords: CARIP-Seq ChIP-Seq Chromatin-associated RNAs Protein-DNA Interactions Embryonic Stem Cells Epigenetics Transcription Factors Epigenetic Regulators Histone Modifications Chromatin Immunoprecipitation Sonication Formaldehyde Cross-linking Glycine Phosphate Buffered Saline Trypsinization MEF Medium Fetal Bovine Serum Sonication Buffer Sodium Dodecyl Sulfate

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

从胚胎干细胞衍生心脏祖细胞

染色质监管机构和各国的全基因组快照<em>爪</em>通过胚胎的ChIP-SEQ

小鼠胚胎干细胞的无血清单层培养神经细胞分化

生成CRISPR / Cas9介导的单等位基因缺失来研究小鼠胚胎干细胞功能增强

心脏分化的表观遗传调控胚胎干细胞和组织

小鼠胚胎Hemogenic内皮细胞的分离

通过高通量抑制剂筛选鉴定调节胚胎干细胞多能性的激酶的简单方法

水平整体安装：厚三维组织横截面皮肤的新型加工和成像方案

人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化

活完好斑马鱼的单细胞 Photoconversion

Read Article