Journal
/
Neuroscience
/
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
JoVE Journal
Neuroscience
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Neuroscience
Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain

DOI:
10.3791/57511-v

•

11:20 min

•

May 07, 2018

•

, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurology, David Geffen School of Medicine,University of California Los Angeles, 3Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Chapters

  • 00:14Title
  • 00:46Preparation of Potassium Ion-selective Microelectrodes
  • 04:18Calibration of Potassium Ion-selective Microelectrodes
  • 05:40Acute Slice Preparation
  • 07:16Measurement of Electrically Evoked K Release
  • 09:26Results: Calibration of Microelectrodes and Measurement of Electrically Evoked K Release
  • 10:53Conclusion

Summary

Automatic Translation

Automatic Translation

Potassium ions contribute to the resting membrane potential of cells and extracellular K+ concentration is a crucial regulator of cellular excitability. We describe how to make, calibrate and use monopolar K+-selective microelectrodes. Using such electrodes enables the measurement of electrically evoked K+ concentration dynamics in adult hippocampal slices.

Tags

Keywords: Potassium Ion Selective MicroelectrodesAdult BrainPotassium Ion HomeostasisBorosilicate Glass CapillariesSilanizationMicroelectrode FabricationDDS Silanization SolutionMicroelectrode PullerPotassium Ion Concentration Measurement

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57511?article_type=v

Focussed Ion Beam Milling and Scanning Electron Microscopy of Brain Tissue
check_url/57511?article_type=v

Subtype-selective Electroporation of Cortical Interneurons
check_url/57511?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/57511?article_type=v

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
check_url/57511?article_type=v

Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
check_url/57511?article_type=v

Preparation of Acute Brain Slices Using an Optimized N-Methyl-D-glucamine Protective Recovery Method
check_url/57511?article_type=v

Effects of Transcranial Alternating Current Stimulation on the Primary Motor Cortex by Online Combined Approach with Transcranial Magnetic Stimulation
check_url/57511?article_type=v

Assessment of Long-term Depression Induction in Adult Cerebellar Slices
check_url/57511?article_type=v

Imaging of Intracellular ATP in Organotypic Tissue Slices of the Mouse Brain using the FRET-based Sensor ATeam1.03YEMK
check_url/57511?article_type=v

Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin

Read Article