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Visualisering af Motor Axon Navigation og kvantificering af Axon Arborization i mus embryoner ved hjælp af lys ark Fluorescens mikroskopi
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1Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Sciences,National Defense Medical Center, 2Institute of Molecular Biology,Academia Sinica, 3Institute of Biotechnology, College of Bio-Resources and Agriculture,National Taiwan University
Chapters
- 00:04Title
- 00:56Fixation and Whole-mount Immunostaining
- 02:55Sample Set-up
- 05:32Quantification of Axon Arborization
- 07:27Results: Visualization and Quantification of Motor Axon Arborization
- 08:22Conclusion
Her, beskriver vi en protokol til at visualisere motorneuron projektion og axon arborization i transgene Hb9::GFP mus embryoner. Efter immunfarvning for motoriske neuroner brugte vi lys ark Fluorescens mikroskopi billede embryoner til efterfølgende kvantitativ analyse. Denne protokol finder anvendelse på andre neuron navigation processer i centralnervesystemet.
