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Dorsalrodsganglier Isolation og primære kultur at studere Neurotransmitter frigivelse
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Neuroscience
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JoVE Journal Neuroscience
Dorsal Root Ganglia Isolation and Primary Culture to Study Neurotransmitter Release

Dorsalrodsganglier Isolation og primære kultur at studere Neurotransmitter frigivelse

DOI:
10.3791/57569-v

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08:15 min

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October 06, 2018

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,
1Graduate Institute of Biomedical Sciences, Department of Physiology and Pharmacology,Chang Gung University, 2Healthy Aging Research Center,Chang Gung University, 3Neuroscience Research Center,Chang Gung Memorial Hospital

Chapters

  • 00:04Title
  • 00:32Isolation of Rat Lumbar DRG
  • 01:40Primary Culture of Rat DRG
  • 04:22Transfection of DRG Cells with NPFFR2 siRNA
  • 05:44Release of Neurotransmitters from Primary DRG Cells
  • 06:42Results: Stimulation-induced Release of Neurotransmitters from Cultured DRG Cells
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

Dorsalrodsganglier (DRG) primære kulturer er ofte bruges til at studere fysiologiske funktioner eller patologi-relaterede begivenheder i sensoriske neuroner. Her, demonstrere vi brugen af lumbal DRG kulturer til at registrere frigivelse af neurotransmittere efter neuropeptid FF receptor type 2 stimulation med en selektiv agonist.

Tags

Keywords: Dorsal Root GangliaPrimary CultureNeurotransmitter ReleaseSensory NeuronsNociceptionRatSpinal CordLumbar DRGCollagenaseTrypsinCentrifugationCell Dissociation

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