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En In Vitro Model til at studere effekten af 5-aminolaevulinsyre-medieret Fotodynamisk terapi på Staphylococcus aureus Biofilm
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JoVE Journal Biology
An In Vitro Model to Study the Effect of 5-Aminolevulinic Acid-mediated Photodynamic Therapy on Staphylococcus aureus Biofilm

En In Vitro Model til at studere effekten af 5-aminolaevulinsyre-medieret Fotodynamisk terapi på Staphylococcus aureus Biofilm

DOI:
10.3791/57604-v

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05:12 min

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April 16, 2018

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, , , , , , ,
1Department of Otorhinolaryngology-Head and Neck Surgery, Eye & ENT Hospital, Shanghai Key Clinical Disciplines of otorhinolaryngology,Fudan University, 2Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Department of Medical Microbiology and Parasitology, School of Basic Medical Science,Shanghai Medical College of Fudan University, 3Department of Dermatology, Huashan Hospital,Fudan University

Chapters

  • 00:04Title
  • 00:40Biofilm Formation and Light Irradiation
  • 02:17Photodynamic Therapy Analysis
  • 04:00Results: Representative Biofilm Viability Analyses
  • 04:33Conclusion

Summary

Automatic Translation

Automatic Translation

Dette manuskript beskriver en protokol for at studere den antimikrobielle effekt af 5-aminolaevulinsyre syre-medieret Fotodynamisk terapi (ALA-PDT) på en Staphylococcus aureus biofilm. Denne protokol kan bruges til at udvikle en in vitro- model for at studere til behandling af bakterielle biofilm med PDT i fremtiden.

Tags

Keywords: 5-aminolevulinic AcidPhotodynamic TherapyStaphylococcus AureusBiofilmIn Vitro ModelAnti-microbial EffectBacterial InfectionCell CultureLight-based TreatmentViable Bacteria Count

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