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Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues
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Developmental Biology
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JoVE Journal Developmental Biology
Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

Organotypic Culture Method to Study the Development Of Embryonic Chicken Tissues

DOI:
10.3791/57619-v

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05:12 min

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August 25, 2018

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1Department of Biology,Mount Saint Vincent University

Chapters

  • 00:00Title
  • 00:51Preparation
  • 01:43Embryo Preparation
  • 02:12Culture Setup
  • 03:36Results: In Vitro Culturing Supports Continued Development
  • 04:46Conclusions

Summary

Automatic Translation

Automatic Translation

Here, we present an organotypic culturing protocol to grow embryonic chicken organs in vitro. Using this method, the development of embryonic chicken tissue can be studied, while maintaining a high degree of control over the culture environment.

Tags

Keywords: Organotypic CultureEye DevelopmentIn-vitro StudyNeural RetinaRetinal Pigmented EpitheliumAutoclavingChick SalineNutrient MediumAntibioticDissectionPetri DishWire MeshFilter Paper

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