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1Department of Internal Medicine-Gastroenterology,University of Michigan, 2Department of Molecular and Integrative Physiology,University of Michigan, 3Department of Molecular, Cellular and Developmental Biology,University of Michigan, 4Department of Gastrointestinal Surgery,The First Affiliated Hospital of Guangxi Medical University, 5Division of Gastroenterology,University of Arizona College of Medicine
Chapters
- 00:04Title
- 00:33Dissection of the Intestinal Segment
- 03:01Removal of the Epithelial Mucosa
- 04:57Collection of Enteric Glial Cells from the Lamina Propria and Submucosa
- 06:23Results: Enteric Glial Cells 24 h After Plating
- 07:12Conclusion
Her beskriver vi isolering av enteric-gliacellene fra intestinal submucosa bruke sekvensiell EDTA incubations for å chelate divalent kasjoner og inkubasjon i ikke-enzymatisk celle gjenopprettingsløsning. Kontaktflate resulterende celle suspensjon på poly-D-lysine og laminin resulterer i en høyanriket kultur av submucosal gliacellene for funksjonsanalyse.
