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Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics
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Immunology and Infection
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JoVE Journal Immunology and Infection
Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

Label-free Neutrophil Enrichment from Patient-derived Airway Secretion Using Closed-loop Inertial Microfluidics

DOI:
10.3791/57673-v

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07:37 min

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June 07, 2018

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, , , , ,
1Research Laboratory of Electronics,Massachusetts Institute of Technology, 2Department of Electrical Engineering and Computer Science,Massachusetts Institute of Technology, 3Department of Medicine,University of Pittsburgh, 4Department of Biological Engineering,Massachusetts Institute of Technology, 5Vascular Medicine Institute,University of Pittsburgh

Chapters

  • 00:04Title
  • 00:40Device Fabrication and Soft Lithography
  • 02:19Tracheal Secretion Collection from Mechanically Ventilated Patients
  • 03:07Experimental Setup
  • 04:27Results: Use of Microfluidics to Obtain Label-free Neutrophil from Patient Clinical Airway Secretions
  • 06:04Conclusion

Summary

Automatic Translation

Automatic Translation

In this research, we demonstrate a label-free neutrophil separation method from clinical airway secretions using closed-loop operation of spiral inertial microfluidics. The proposed method would expand the clinical in vitro assays for various respiratory diseases.

Tags

Keywords: Label-freeNeutrophil EnrichmentPatient-derivedAirway SecretionClosed-loopInertial MicrofluidicsPulmonary DiseasesPneumoniaAsthmaCystic FibrosisCOPDPDMSMicro MachineMoldPetri DishVacuum DesiccatorAir PlasmaPhosphate Buffered SalineCell StrainerSpiral Micro ChannelsLuer Connector

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