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Chapters
- 00:05Title
- 00:46Maintaining and Preparing the Flies
- 02:52Bacterial Culture
- 04:15Orally Infecting Flies
- 06:18Measuring Bacterial Load and Bacterial Shedding
- 07:18Results: P. aeruginosa and P. entomophila Infections
- 08:43Conclusion
This protocol describes methods to orally expose and infect the fruit fly Drosophila melanogaster with bacterial pathogens, and to measure the number of infectious bacteria shed following gut infection. We further describe the effect of immune mutants on fly survival following oral bacterial infection.
