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Homochronic Transplantation af Interneuron prækursorer i tidlige Postnatal mus hjerner
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Neuroscience
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JoVE Journal Neuroscience
Homochronic Transplantation of Interneuron Precursors into Early Postnatal Mouse Brains

Homochronic Transplantation af Interneuron prækursorer i tidlige Postnatal mus hjerner

DOI:
10.3791/57723-v

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10:08 min

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June 08, 2018

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, , ,
1Kavli Institute for Systems Neuroscience and Centre for Neural Computation,Norwegian University of Science and Technology, 2Eunice Kennedy Shriver National Institute of Child Health and Human Development,National Institutes of Health

Chapters

  • 00:04Title
  • 00:57Striatum, Hippocampus, and Cortex Harvest: Technique 1
  • 03:15Striatum, Hippocampus, and Cortex Harvest: Technique 2
  • 04:33Generation of Single Cell Suspensions
  • 06:01P0-2 Wild-Type (WT) Pup Transplantation
  • 08:13Results: Representative Grafted Interneuron Analyses
  • 09:28Conclusion

Summary

Automatic Translation

Automatic Translation

Udfordrende unge neuroner i nye områder af hjernen kan afsløre vigtige indsigt i hvordan miljøet sculpts neuronal skæbne og modning. Denne protokol beskriver en procedure for at høste interneuron prækursorer fra specifikke hjerneregioner og omplante dem enten homotopically eller heterotopically i hjernen hos postnatal unger.

Tags

Keywords: Homochronic TransplantationInterneuron PrecursorsPostnatal Mouse BrainsDevelopmental NeuroscienceCell HarvestingCell GraftingStriatumHippocampusFluorescent Reporter MouseArtificial Cerebrospinal FluidDissecting MicroscopeForceps

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