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रेटिना क्रायो-वर्गों, पूरे mounts, और Hypotonic Microvascular Pericytes के Immunohistochemical दृश्य के लिए अलग Vasculature तैयारी
JoVE Journal
Biology
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JoVE Journal Biology
Retinal Cryo-sections, Whole-Mounts, and Hypotonic Isolated Vasculature Preparations for Immunohistochemical Visualization of Microvascular Pericytes
DOI:

10:46 min

October 07, 2018

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Chapters

  • 00:04Title
  • 00:41Cryosection Rat Retinal Tissue Preparation and Immunostaining
  • 03:23Whole Mount Rat Retinal Tissue Preparation and Immunostaining
  • 05:08Hypotonic Isolation of Ray Retinal Tissue Preparation and Immunostaining
  • 08:27Results: Representative Immunohistochemistry Images
  • 10:18Conclusion

Summary

Automatic Translation

हम चूहे रेटिना microvascular pericytes के immunohistochemical दृश्य के लिए तीन अलग ऊतक तैयार करने की तकनीक का प्रदर्शन, यानी, क्रायो-वर्गों, पूरे माउंट, और संवहनी नेटवर्क के hypotonic अलगाव.

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