Journal
/
Bioengineering
/
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D
JoVE Journal
Bioengineering
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Bioengineering
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

DOI:
10.3791/57739-v

•

11:46 min

•

May 19, 2018

•

, , ,
1Department of Bioengineering,Stanford University, 2Department of Materials Science and Engineering,Stanford University

Chapters

  • 00:04Title
  • 00:58ELP Expression Protocol
  • 05:06Cell Encapsulation in 3D Elastin-like Protein Hydrogels
  • 07:11Immunocytochemistry of Cells in 3D ELP Hydrogels
  • 09:47Results: Purification of Elastin-like Protein and its Application for 3D Hydrogel Cell Encapsulation
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

Recombinant protein-engineered hydrogels are advantageous for 3D cell culture as they allow for complete tunability of the polymer backbone and therefore, the cell microenvironment. Here, we describe the process of recombinant elastin-like protein purification and its application in 3D hydrogel cell encapsulation.

Tags

Keywords: Elastin-like ProteinHydrogel3D Cell EncapsulationFluorescent LabelingConfocal MicroscopyBiomaterialsRegenerative MedicineCell BiologyCell-extracellular Matrix InteractionsRecombinant Protein ExpressionIPTG InductionCell PelletTEN Buffer

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57739?article_type=v

A Method for Ovarian Follicle Encapsulation and Culture in a Proteolytically Degradable 3 Dimensional System
check_url/57739?article_type=v

Ordering Single Cells and Single Embryos in 3D Confinement: A New Device for High Content Screening
check_url/57739?article_type=v

Production, Characterization and Potential Uses of a 3D Tissue-engineered Human Esophageal Mucosal Model
check_url/57739?article_type=v

Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
check_url/57739?article_type=v

Improved 3D Hydrogel Cultures of Primary Glial Cells for In Vitro Modelling of Neuroinflammation
check_url/57739?article_type=v

Flash NanoPrecipitation for the Encapsulation of Hydrophobic and Hydrophilic Compounds in Polymeric Nanoparticles
check_url/57739?article_type=v

Measuring Global Cellular Matrix Metalloproteinase and Metabolic Activity in 3D Hydrogels
check_url/57739?article_type=v

Controlled Strain of 3D Hydrogels under Live Microscopy Imaging
check_url/57739?article_type=v

Light-Controlled Fermentations for Microbial Chemical and Protein Production
check_url/57739?article_type=v

Large-Scale, Automated Production of Adipose-Derived Stem Cell Spheroids for 3D Bioprinting

Read Article