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Produktionen af Elastin-lignende Protein Hydrogels til indkapsling og immunfarvning af celler i 3D
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Bioengineering
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JoVE Journal Bioengineering
Production of Elastin-like Protein Hydrogels for Encapsulation and Immunostaining of Cells in 3D

Produktionen af Elastin-lignende Protein Hydrogels til indkapsling og immunfarvning af celler i 3D

DOI:
10.3791/57739-v

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11:46 min

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May 19, 2018

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, , ,
1Department of Bioengineering,Stanford University, 2Department of Materials Science and Engineering,Stanford University

Chapters

  • 00:04Title
  • 00:58ELP Expression Protocol
  • 05:06Cell Encapsulation in 3D Elastin-like Protein Hydrogels
  • 07:11Immunocytochemistry of Cells in 3D ELP Hydrogels
  • 09:47Results: Purification of Elastin-like Protein and its Application for 3D Hydrogel Cell Encapsulation
  • 11:18Conclusion

Summary

Automatic Translation

Automatic Translation

Rekombinante protein-manipuleret hydrogels er fordelagtige for 3D cellekultur, som de giver mulighed for komplet tunability af polymer rygrad og derfor celle mikromiljø. Her, beskriver vi processen med rekombinant elastin-lignende protein oprensning og dens anvendelse i 3D hydrogel celle indkapsling.

Tags

Elastin-like ProteinHydrogel3D Cell EncapsulationFluorescent LabelingConfocal MicroscopyBiomaterialsRegenerative MedicineCell BiologyCell-extracellular Matrix InteractionsRecombinant Protein ExpressionIPTG InductionCell PelletTEN Buffer

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