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FM farvestof cykling på Synapse: sammenligning af høje kalium depolarisering, elektriske og Channelrhodopsin Stimulation
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Neuroscience
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JoVE Journal Neuroscience
FM Dye Cycling at the Synapse: Comparing High Potassium Depolarization, Electrical and Channelrhodopsin Stimulation

FM farvestof cykling på Synapse: sammenligning af høje kalium depolarisering, elektriske og Channelrhodopsin Stimulation

DOI:
10.3791/57765-v

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08:31 min

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May 24, 2018

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1Department of Biological Sciences,Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development,Vanderbilt University and Medical Center

Chapters

  • 00:04Title
  • 00:42Larval Glue Dissection
  • 02:36Electrical Stimulation: FM Dye Loading and Unloading
  • 04:08Channelrhodopsin Stimulation: FM Dye Loading and Unloading
  • 05:21Fluorescence Quantification
  • 06:12Results: Synaptic Ultrastructure with FM Dye Photoconversion to Mark Vesicles
  • 07:22Conclusion

Summary

Automatic Translation

Automatic Translation

Synaptic vesikel (SV) cykling er den centrale mekanisme for intercellulær kommunikation på neuronal synapser. FM farvestof optagelse og udgivelse er det primære middel til kvantitativt boltpistoler SV endo- og exocytose. Her, sammenligner vi alle stimulation metoderne til at drive FM1-43 cykling på Drosophila neuromuskulære junction (NMJ) model synapse.

Tags

FM DyeSynaptic Vesicle CyclingNeuromuscular JunctionDrosophila LarvaeHigh Potassium DepolarizationElectrical StimulationOptogeneticsAnti-HRP AntibodyElastomer-coated CoverslipDissectionGluingMotor NerveCalcium-free SalineSuction PipetteMicroelectrode PullerMicro ForgeMicromanipulator

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