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Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model
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Immunology and Infection
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JoVE Journal Immunology and Infection
Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

Quantifying Vibrio cholerae Colonization and Diarrhea in the Adult Zebrafish Model

DOI:
10.3791/57767-v

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08:03 min

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July 12, 2018

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, , ,
1Department of Microbiology, Immunology, and Biochemistry,Wayne State University School of Medicine

Chapters

  • 00:04Title
  • 00:40Inoculating Zebrafish with V. cholerae
  • 01:49Collecting Intestines and Assessing Bacterial Colonization
  • 04:27Metrics for Fish Diarrhea: Mucin Levels, Protein Levels, A600 and Excreted Bacteria
  • 06:43Results: Colonization and Diarrhea, 18 h After a 6 h Infection Period
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

Zebrafish are a natural Vibrio cholerae host and can be used to recapitulate and study the entire infectious cycle from colonization to transmission. Here, we demonstrate how to assess V. cholerae colonization levels and quantify diarrhea in zebrafish.

Tags

Keywords: Vibrio CholeraeZebrafish ModelColonizationDiarrheaIntestinal MicrobiotaLife CycleInfectionDiagnosticDissectionIntestinal Tract

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