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JoVE Journal Cancer Research
Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System
 

Live Cell Imaging of the TGF- β/Smad3 Signaling Pathway In Vitro and In Vivo Using an Adenovirus Reporter System

Article DOI: 10.3791/57926-v • 11:06 min • July 30th, 2018
July 30th, 2018 •
*1, *1, 1, 1
1Department of Surgery (RMH), University of Melbourne
* These authors contributed equally

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Summary
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Here, we present a protocol for live cell imaging of TGF-β/Smad3 signaling activity using an adenovirus reporter system. This system tracks transcriptional activity in real-time and can be applied to both single cells in vitro and in live animalmodels.

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Keywords: Live Cell Imaging TGF-β/Smad3 Signaling Pathway Adenovirus Reporter System Cancer Cell Lines Breast Cancer Wound Healing Assay MDA-MB-231 Cells Adenovirus Infection Fluorescence Microscopy GFP Td-Tomato TGF-beta ImageJ
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