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Pancreas Islet indlejring for paraffinsnit
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JoVE Journal Biology
Pancreatic Islet Embedding for Paraffin Sections

Pancreas Islet indlejring for paraffinsnit

DOI:
10.3791/57931-v

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09:09 min

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June 29, 2018

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, , , ,
1Diabetes Center of Excellence,UMass Medical School, 2Department of Medicine,Saint Vincent Hospital, 3Department of Pathology, Morphology Core,UMass Medical School

Chapters

  • 00:04Title
  • 00:35Islet Fixation
  • 02:06Preparation of Islet Disc
  • 05:55Results: Paraffin Section Analysis
  • 07:24Conclusion

Summary

Automatic Translation

Automatic Translation

Ex vivo i bugspytkirtlen islet undersøgelser er vigtige for diabetes research. Eksisterende teknikker til at studere kulturperler Holme i deres native 3-dimensionelle arkitektur er tidskrævende, ineffektiv og sjældent brugt. Dette arbejde beskriver en ny, enkel og effektiv metode for at skabe høj kvalitet paraffinsnit af hele kulturperler Holme.

Tags

Pancreatic IsletParaffin SectionsEmbedding MethodIslet EquivalentsPBS WashFixativeAgarose Blue BeadsMicropipette TipCentrifugation

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