A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, 2Department of Medicine,Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne
Chapters
- 00:04Title
- 01:01Reverse Transcription
- 01:50Droplet Generation and Digital PCR
- 03:10Droplet Reading and Analyzing
- 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
- 04:10Conclusion
Circulating microRNAs have shown promise as biomarkers for cardiovascular diseases and acute myocardial infarctions. In this study, we describe a protocol for miRNA extraction, reverse transcription, and digital PCR for the absolute quantification of miRNAs in the serum of patients with cardiovascular disease.
