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1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, 2Department of Medicine,Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne
Chapters
- 00:04Title
- 01:01Reverse Transcription
- 01:50Droplet Generation and Digital PCR
- 03:10Droplet Reading and Analyzing
- 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
- 04:10Conclusion
Cirkulerende MicroRNA har vist lovende som biomarkører for hjerte-kar-sygdomme og akut myokardieinfarkt hjertet. I denne undersøgelse beskriver vi en protokol for miRNA udvinding, reverse transkription og digital PCR for den absolutte kvantificering af miRNAs i serum hos patienter med hjerte-kar-sygdom.
