A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Department of Cardiology and Angiology I, Heart Center Freiburg University, Faculty of Medicine,University of Freiburg, 2Department of Medicine,Monash University, 3Department of Medicine I, Lighthouse Core Facility, Medical Center,University of Freiburg, 4Department of Cardiology, Augustinerinnen Hospital, Academic Teaching Hospital,University of Cologne
Chapters
- 00:04Title
- 01:01Reverse Transcription
- 01:50Droplet Generation and Digital PCR
- 03:10Droplet Reading and Analyzing
- 03:37Results: miR-499 a Possible Biomarker to Differentiate Patients with CAD and a STEMI
- 04:10Conclusion
Sirkulerende microRNAs har vist lovende som biomarkers for kardiovaskulære sykdommer og akutt myokardisk infarkt. I denne studien beskriver vi en protokoll for miRNA utvinning, omvendt transkripsjon og digital PCR for den absolutte kvantifiseringen av miRNAs i serum av pasienter med hjerte-og karsykdommer.
