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Generation og On-Demand indledning af akut Ictal aktivitet i gnaver og menneskeligt væv
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1Division of Fundamental Neurobiology,Krembil Research Institute, 2Institute of Medical Science, Faculty of Medicine,University of Toronto, 3Institute of Biomaterials and Biomedical Engineering,University of Toronto, 4Division of Neurosurgery, Department of Surgery,University of Toronto, 5Division of Neurology, Department of Medicine,University of Toronto, 6Department of Physiology,University of Toronto
Chapters
- 00:04Title
- 00:46Preparation of Cortical Slices from the Somatosensory-motor Cortex
- 02:40Electrophysiological Recordings of the Superficial Cortical Layer and Seizure Induction
- 04:43Results: Acute In Vitro 4-AP Cortical Seizure Model and Ictal Events Generated by Optogenetics
- 06:19Conclusion
Akutte anfald modeller er vigtige for at studere de underliggende epileptiform begivenheder mekanismer. Desuden giver evnen til at generere epileptiform begivenheder på efterspørgsel en meget effektiv metode til at studere den nøjagtige rækkefølge af begivenheder ligger til grund for deres indledning. Her beskriver vi de akutte 4-aminopyridine kortikale beslaglæggelse modeller etableret i mus og humant væv.
