Journal
/
Biology
/
Laktat Dehydrogenase lagring analysen-En enkel og pålitelig metode å avgjøre Bulk Autophagic lagring aktivitet i pattedyrceller
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
The Lactate Dehydrogenase Sequestration Assay — A Simple and Reliable Method to Determine Bulk Autophagic Sequestration Activity in Mammalian Cells

Laktat Dehydrogenase lagring analysen-En enkel og pålitelig metode å avgjøre Bulk Autophagic lagring aktivitet i pattedyrceller

DOI:
10.3791/57971-v

•

09:34 min

•

July 27, 2018

•

, ,
1The Autophagy Team, Centre for Molecular Medicine Norway (NCMM), Nordic EMBL Partnership,University of Oslo

Chapters

  • 00:04Title
  • 00:40Cell Seeding and Treatment
  • 02:46Cell Harvest and Preparation for Electrodisruption
  • 03:53Plasma Membrane Electrodisruption and Separation of Sedimentable- and Total Cell Fractions
  • 05:50LDH Extraction and Measurement of LDH Enzymatic Activity
  • 06:55Results: Monitoring Bulk Autophagic Sequestration Activity in Mammalian Cells
  • 08:43Conclusion

Summary

Automatic Translation

Automatic Translation

Her er en enkel og godt validert protokoll for å måle bulk autophagic lagring aktivitet i pattedyrceller beskrevet. Metoden er basert på kvantifisere andelen av laktat dehydrogenase (LDH) i sedimentable celle fraksjoner sammenlignet med totale mobilnettet LDH nivåer.

Tags

Keywords: Lactate Dehydrogenase Sequestration AssayAutophagyAutophagic SequestrationCell CultureTrypsinCell CountingCell SeedingBafilomycin A1Cell Detachment

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/57971?article_type=v

Pyrosequencing: A Simple Method for Accurate Genotyping
check_url/57971?article_type=v

Use of the Protease Fluorescent Detection Kit to Determine Protease Activity
check_url/57971?article_type=v

Clonogenic Assay: Adherent Cells
check_url/57971?article_type=v

A Matrigel-Based Tube Formation Assay to Assess the Vasculogenic Activity of Tumor Cells
check_url/57971?article_type=v

En enkel metode for Imaging Arabidopsis Gir hjelp Perfluorodecalin som et infiltrerende Imaging Medium
check_url/57971?article_type=v

Analyse av Cell Cycle Posisjon i pattedyrceller
check_url/57971?article_type=v

Autonomt Bioluminescent mammalieceller for Kontinuerlig og sanntids overvåking av Cytotoksisitet
check_url/57971?article_type=v

Enkelt Bulk Avlesning av digitale Nukleinsyre Kvantifisering Analyser
check_url/57971?article_type=v

Nyreskade Mikroinjeksjon i Sebrafisk til Model Akutt nyreskade
check_url/57971?article_type=v

Filtrering Isolering av nukleinsyrer: En enkel og Rapid DNA Extraction Method

Read Article